RNA

Part:BBa_K5179300:Design

Designed by: Caden Sanko   Group: iGEM24_William-and-Mary   (2024-10-01)


P4 CI RNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 50
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 53
    Illegal BsaI.rc site found at 47


Design Notes

None.


Source

The CI RNA is and CI RNA dependent terminators are from the satellite phage P4.

References

Forti, F., Dragoni, I., Briani, F., Dehò, G., & Ghisotti, D. (2002). Characterization of the small antisense CI RNA that regulates bacteriophage P4 immunity. Journal of molecular biology, 315(4), 541–549.

Gruber AR, Lorenz R, Bernhart SH, Neuböck R, Hofacker IL. The Vienna RNA Websuite. Nucleic Acids Research, Volume 36, Issue suppl_2, 1 July 2008, Pages W70-W74, DOI: 10.1093/nar/gkn188

Sabbattini, P., Forti, F., Ghisotti, D., & Dehò, G. (1995). Control of transcription termination by an RNA factor in bacteriophage P4 immunity: identification of the target sites. Journal of bacteriology, 177(6), 1425–1434.